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KMID : 0903519860290010073
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1986 Volume.29 No. 1 p.73 ~ p.77
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Effects of Auxin , GA and Cytokinin on the Protein Synthesis(Accumulation) of Soybean
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Abstract
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Aqueous solutions of 2, 4-D, BA or GA₃(10^(-6), 10^(-5), and 10^(-4)M, respectively) were sprayed onto soybean (Glycine max) plants in the flowering stage, and proteins of immature (33days after flowering) and mature (77days after flowering) seeds were analyzed by electrophoresis to elucidate the effects of the growth reguators on protein synthesis or protein accumulation in the seeds. Accumulations of some proteins were altered by 2, 4-D or BA at certain concentrations, but no proteins were affected by GA₃. The α and α´ subunits of 7S and acidic subunit of 11S disappeared in mature seeds after treatments at the flowering stage with 2, 4-D or BA. The presence of α and α´ subunits of 7S and acidic subunit of 11S in immature seeds indicated that the absence of the above polypeptides in mature seeds did not result from inhibitions in syntheses of the polyeptides by the growth regulators. Disappearance of the above proteins in mature seeds seemed to be concerned with the action of specific proteolytic enzymes) (metalloendopeptidase?), and 2, 4-D and BA might promote gene expression or activation of the enzyme.
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